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Tiny_Rat t1_j81m6ud wrote

If they autoclave it, a sealed vessel will still explode, as the pressure inside the vessel and the machine will still change at different rates. Plus, killing the bacteria won't make the solution less cloudy, it will just be cloudy with dead bacteria instead of living ones. And, worst of all, heat treatment will most likely kill the fluorescent proteins anyways.


RebelWithoutAClue t1_j82asnn wrote

It could be autoclaved in a pressure cooker if the protein can tolerate the temp.

In a pressure cooker the pressure is also exerted on the outside of the ampule. No pressure differential, no kaboom.