Submitted by AutomaticAd1918 t3_z67gnl in askscience
Cleistheknees t1_iy0u6dt wrote
Reply to comment by FogeltheVogel in How exactly does CRISPR-CAS9 insert new genes? by AutomaticAd1918
Preface: the last “i” in HITI is actually for “integration”, not “insertion” as stated above, in case you wanted to google around for more info.
You guide the process in the same way. The donor sequence used (theoretically used, anyways) in HITI is capped by the same sequences that gRNA-Cas9 is pointed at, and in fact is exceptionally accurate. The idea behind HITI is that it isn’t limited to actively differentiating cells.
[deleted] t1_iy1m362 wrote
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