Almost certainly, because they would recognize basic parts of the anatomy that they could see or feel on their own / other peoples' bodies, such as arms and legs.

The human body is also really good (almost too good) at picking out faces, so even if you showed someone who had never seen anime before a character like Yugi Muto who has massive eyes, hair not typically seen on a person, and is often seen carrying weird devices on his arm, they'd be able to work out it was human.

You could take this question further by thinking about some hypotheticals. For example, if a person was chained up, facing a solid wall for their entire life (similar to Plato's Cave), and the only things they saw were the shadows of other humans walking past, would the person be able to identify a human by looking at them, based on what features they saw on the shadows? Or would they see the shadow and the person as two separate beings?