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ddlbot t1_j9dyrn1 wrote

me too, i asked ChatGPT to summarize it...


The article discusses the challenges of quantifying ultrastructural features of pleomorphic organelles in three dimensions using cellular cryo-electron tomography (cryo-ET) and proposes a semiautomated workflow to segment organellar membranes and reconstruct their underlying surface geometry in cryo-ET. The authors also developed an open-source suite of ultrastructural quantifications, integrated into a single pipeline called the surface morphometrics pipeline, which enables rapid modeling of complex membrane structures and allows detailed mapping of inter- and intramembrane spacing, curvedness, and orientation. The authors demonstrate the advantages of this approach by combining cryo-ET with cryo-fluorescence microscopy to correlate bulk mitochondrial network morphology with membrane ultrastructure of individual mitochondria in the presence and absence of endoplasmic reticulum (ER) stress. Using their pipeline, the authors demonstrate ER stress promotes adaptive remodeling of ultrastructural features of mitochondria and suggest that changes in membrane ultrastructure correlate to mitochondrial network morphologies. Overall, the pipeline offers opportunities for quantifying changes in membrane ultrastructure on a single-cell level using cryo-ET, opening new opportunities to define changes in ultrastructural features induced by diverse types of cellular perturbations


so i said "Explain like i'm Five"


>Scientists want to see how tiny parts inside cells look like in 3D to understand how cells work. They use a fancy machine called cryo-electron tomography (cryo-ET) to take pictures of these tiny parts. But it's hard to understand what the pictures mean. So, the scientists came up with a way to make the pictures easier to understand. They made a computer program that helps them to see the parts better and to compare them with other cells. They used this program to study mitochondria, tiny parts that make energy for cells. They found that when cells are stressed, mitochondria change their shape and structure to adapt to the stress. They hope that this program will help them learn more about how cells work and how they respond to different conditions.


cleare7 t1_j9eol61 wrote

Very cool - another step to better understanding how cells work. Thanks for the ELI5.