>The oyster mushroom (Pleurotus ostreatus) is a staple of many kinds of cuisine, prized for its mild flavors and a scent vaguely hinting at anise. These cream-colored mushrooms are also one of several types of carnivorous fungi that prey on nematodes (roundworms) in particular. The mushrooms have evolved a novel mechanism for paralyzing and killing its nematode prey: a toxin contained within lollipop-like structures called toxocysts that, when emitted, cause widespread cell death in roundworms within minutes. Scientists have now identified the specific volatile organic compound responsible for this effect, according to a new paper published in the journal Science Advances. > >Carnivorous fungi like the oyster mushroom feed on nematodes because these little creatures are plentiful in soil and provide a handy protein source. Different species have evolved various mechanisms for hunting and consuming their prey. For instance, oomycetes are fungus-like organisms that send out "hunter cells" to search for nematodes. Once they find them, they form cysts near the mouth or anus of the roundworms and then inject themselves into the worms to attack the internal organs. Another group of oomycetes uses cells that behave like prey-seeking harpoons, injecting the fungal spores into the worm to seal its fate. > >Other fungi produce spores with irritating shapes like stickles or stilettos. The nematodes swallow the spores, which get caught in the esophagus and germinate by puncturing the worm's gut. There are sticky branch-like structures that act like superglue; death collars that detach when nematodes swim through them, injecting themselves into the worms; and a dozen or so fungal species employ snares that constrict in under a second, squeezing the nematodes to death. > >The oyster mushroom eschews these physical traps in favor of a chemical mechanism. P. ostreatus is what's known as a "wood rotter" that targets dead trees, but wood is relatively poor in protein. Its long branching filaments (called hyphae) are the part of the 'shroom that grows into the rotting wood. Those hyphae are home to the toxocysts. When nematodes encounter the toxocysts, the cysts burst, and the nematodes typically become paralyzed and die within minutes. Once the prey is dead, the hyphae grow into the nematode bodies, dissolving the contents and absorbing the slurry for the nutrients. > >... > >But Lee et al. could not identify the specific toxins responsible for the effect, though they did note that the oyster mushroom's chemical mechanism was distinct from the nematicides currently used to control nematode populations. For the new study, Lee and co-authors used gas chromatography-mass spectrometry to do just that. The first version of the experiment tested a vial sample containing just the culture medium and glass beads. A second version tested a vial sample containing P. ostreatus that had been cultured for two to three weeks. The third version was a combination of the first two, testing a vial sample that contained both cultured P. ostreatus and glass beads. > >The culprit: a volatile ketone called 3-octanone, one of several naturally occurring volatile organic compounds (VOCs) that fungi use for communication. It seems 3-octanone also serves as a potent nematode-killing mechanism. Exposing four species of nematode to 3-octanone triggered the telltale massive (and fatal) influx of calcium ions into nerve and muscle cells. The dosage is critical, per the authors. Low dosages are a repellant to slugs and snails, but high dosages are fatal. The same is true for nematodes. A high concentration of more than 50 percent of 3-octanone is required to trigger the rapid paralysis and widespread cell death. The team also induced thousands of random genetic mutations in the fungus. Those mutants that didn't develop toxocysts on their hyphae were no longer toxic to the nematode Caenorhabditis elegans.

Some super interesting mycological research here on carnivorous fungi and a chemical mechanism that is used in lieu of physical ones.