This is usually true in most cases, but it can be done. I study a relatively small enzyme (~15 kDa) in my research, and there is a paper describing a method to synthetically make the entire enzyme. They even tested the activity of their synthetic enzyme and found it was basically identical to biochemically produced enzyme.
That said, you won’t find me using solid phase peptide synthesis to make this enzyme - E. coli are far better at it than I am!
Spyro_ t1_irbqxsm wrote
Reply to comment by JigglymoobsMWO in is it possible to synthesize proteins chemically? by yeeturking
> We can make peptides but not proteins.
This is usually true in most cases, but it can be done. I study a relatively small enzyme (~15 kDa) in my research, and there is a paper describing a method to synthetically make the entire enzyme. They even tested the activity of their synthetic enzyme and found it was basically identical to biochemically produced enzyme.
That said, you won’t find me using solid phase peptide synthesis to make this enzyme - E. coli are far better at it than I am!