fertthrowaway t1_iy1s8at wrote on November 28, 2022 at 2:19 AM Reply to comment by inblue01 in How exactly does CRISPR-CAS9 insert new genes? by AutomaticAd1918 With repair template and active enough homologous recombination, you can get like 99-100% correct edit efficiency in many organisms. If you rely on NHEJ after the nuclease cut, then you basically have to screen through repaired DNA craters though, yeah. Permalink Parent 2
fertthrowaway t1_iy1s8at wrote
Reply to comment by inblue01 in How exactly does CRISPR-CAS9 insert new genes? by AutomaticAd1918
With repair template and active enough homologous recombination, you can get like 99-100% correct edit efficiency in many organisms.
If you rely on NHEJ after the nuclease cut, then you basically have to screen through repaired DNA craters though, yeah.