BLAST is an online sequence alignment tool by NCBI.
I am sequencing the genome of the Dengue virus itself that we extract from an infected person. And we are able to determine the serotype affecting a person via real time PCR itself. So we draw the patient's blood, spin it down into serums, then we do nucleic acid extraction to obtain the RNA. Then we do real time RT-PCR to detect the presence of any sereotype of the virus.
I think this the interesting and crucial point. Are serotypes mainly influenced by genotypes or are there epigenetic factors at play also.
I have done many Dengue E1 Sequencing and I frequently BLAST the sequences. We do see that these sequences can be catergorised into the serotypes themselves. So does mean that the sequences, and therefore genotype, confers the serotypes?
tanteidaiko OP t1_iz42y1y wrote
Reply to comment by Bitchwar in What are Serotypes? Are they a kind of Phenotype / Genotype? by tanteidaiko
BLAST is an online sequence alignment tool by NCBI.
I am sequencing the genome of the Dengue virus itself that we extract from an infected person. And we are able to determine the serotype affecting a person via real time PCR itself. So we draw the patient's blood, spin it down into serums, then we do nucleic acid extraction to obtain the RNA. Then we do real time RT-PCR to detect the presence of any sereotype of the virus.